Chapter 4: Advanced Techniques of DNA Science

Limitations of Basic Techniques

The Biochemist's Problem

The Molecular Biologist's Solution

Vectors Used in Making Genomic Libraries

The l Genomic Library

Screening a Genomic Library

The Uniqueness of DNA Sequences

Identifying Specific DNA Sequences

Screening a Genomic Library by Plaque Hybridization

Making a Probe for the ß Globin Gene

Restriction Mapping and Subcloning

Constructing a cDNA Library

Constructing and Screening an Expression Library

Monoclonal Antibodies

Screening a cDNA library with an Oligonucleotide Probe

Determining Sequence of Cloned DNA

Interpreting DNA Sequence

Southern and Northern Hybridization

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