The propagation (cloning) of a recombined plasmid cannot occur in vitro.  As a consequence, the bacterium Escherichia coli is commonly used for this process.  The reasons this cell is ideal are Bacterial Population Growth in a Closed Environment (Batch Culture)


 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

1. Lag Phase - cells first introduced; adjust to the environment.
2. Log Phase - cells reproduce exponentially.
3. Stationary Phase - cells reach the upper limit of population growth.
4. Decline Phase - cell population declines exponentially as resources are
                           depleted.