Chapter 3: Basic Tools and Techniques of DNA Science

restriction endonucleases
restriction fragments
restriction map

The First Recombinant DNA Molecules
Paul Berg
"sticky ends"
Stanley Cohen
Herb Boyer

Restriction Endonucleases
enzyme classes
Type I
Type II
Type III

Agarose-Gel Electrophoresis

DNA Recombination and Ligation

The Host Cell: The Bacterium Escherichia coli

The Plasmid Vector

Induced Transformation

Mechanism of DNA Uptake

Selection of Transformants

Isolation and Analysis of Recombinant Plasmids

Table of Contents