Chapter 3: Basic Tools and Techniques of DNA Science

Restriction-Modification
restriction endonucleases
methylation
restriction fragments
restriction map

The First Recombinant DNA Molecules
Paul Berg
"sticky ends"
Stanley Cohen
Herb Boyer

Restriction Endonucleases
enzyme classes
Type I
Type II
Type III

Agarose-Gel Electrophoresis

DNA Recombination and Ligation

The Host Cell: The Bacterium Escherichia coli

The Plasmid Vector

Induced Transformation

Mechanism of DNA Uptake

Selection of Transformants

Isolation and Analysis of Recombinant Plasmids

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